Faculty Profiles

Wagh, S. B., Berthold, D., Majeed, I., Lewis, L., Schilter, D., Mertens, B., … Kornienko, A. V. (2024). Sphaeropsidin A C15-C16 Cross-Metathesis Analogues with Potent Anticancer Activity. ChemMedChem, e202400288. https://doi.org/10.1002/cmdc.202400288

Sowersby, D. S., & Lewis, L. K. (2023). SURE gel electrophoresis: a method for concentrating dilute DNA samples directly within gels. Analytical Biochemistry, 684, 115373.

Holland, C. L., Weis, M. F., England, C. J., Berry, A. M., Hall, P. D., & Lewis, L. K. (2023). Deficiency in homologous recombination is associated with changes in cell cycling  and morphology in Saccharomyces cerevisiae. Experimental Cell Research, 430, 113701.

Malla, S. R. L., Gujjari, A., Corona, C. E., Beall, G. W., & Lewis, L. K. (2023). Spectrophotometric and nucleic acid-binding properties of  halloysite clay nanotubes and kaolinite. Heliyon, 9, e13009. Retrieved from https://www.sciencedirect.com/science/article/pii/S2405844023002165?via%3Dihub

Fitzgerald, O. R., Rodriguez, N. D., & Lewis, L. K. (2022). High efficiency plasmid DNA transformation in yeast. Methods in Molecular Biology, 2513, 15–22. Retrieved from https://link.springer.com/protocol/10.1007/978-1-0716-2399-2_2

Holland, C. L., Sanderson, B. A., Titus, J. K., Weis, M. F., Riojas, A. M., Malczewskyj, E., … Lewis, L. K. (2021). Suppression of telomere capping defects of Saccharomyces cerevisiae yku70 and yku80 mutants by telomerase. Genes/Genomes/Genetics, 11(12), jkab359.

England, C. J., Gray, T. C., Malla, S. R. L. R., Oliveira, S. A., Martin, B. R., Beall, G. W., & Lewis, L. K. (2020). pH-dependent sedimentation of DNA in the presence of divalent, but not monovalent, metal ions. Analytical Biochemistry, 616, 114099.

Ghanem, N. Z., Malla, S. R. L., Araki, N., & Lewis, L. K. (2019). Quantitative assessment of changes in morphology, cell division number and growth arrest during telomere-initiated senescence in Saccharomyces cerevisiae. Experimental Cell Research, 381, 18–28.

Ream, J. A., Lewis, L., & Lewis, K. A. (2018). Horizontal Agarose Gel Mobility Shift Assay for Protein-RNA Complexes. Methods in Molecular Biology, 1855, 363–370. https://doi.org/10.1007/978-1-4939-8793-1_31

Gujjari, A., Rodriguez, B. V., Pescador, J., Maeder, C., Beall, G. W., & Lewis, L. K. (2017). Factors affecting the association of single- and double-stranded RNAs with montomorillonite nanoclays. International Journal of Biological Macromolecules, 109, 551–559. Retrieved from https://www.ncbi.nlm.nih.gov/pubmed/29277420

Wood, W. N., Smith, K. D., Ream, J. A., & Lewis, L. K. (2016). Enhancing yields of low and single copy number plasmid DNAs from Escherichia coli cells. Journal of Microbiological Methods.

Ream, J. A., Lewis, L. K., & Lewis, K. A. (2016). Rapid agarose gel electrophoretic mobility shift assay for quantitating protein:RNA interactions. Analytical Biochemistry, 511, 36–41. Retrieved from 10.1016/j.ab.2016.07.027

Lewis, L., Rodriguez, B. V., Malczewskyj, E. T., Cabiya, J. M., & Maeder, C. (2015). Identification of an RNase-resistant population of RNAs in Saccharomyces cerevisiae extracts: separation from chromosomal DNA by selective precipitation.

Rodriguez, B. V., Pescador, J., Pollok, N., Beall, G. W., Maeder, C., & Lewis, L. K. (2015). Impact of size, secondary structure, and counterions on the binding of small ribonucleic acids to layered double hydroxide nanoparticles. Biointerphases, 10(4), 041007. https://doi.org/10.1116/1.4936393

Sanderson, B. A., Araki, N., Lilley, J. L., Guerrero, G., & Lewis, L. (2014). Modification of gel architecture and TBE/TAE buffer composition to minimize heating during agarose gel electrophoresis. Analytical Biochemistry, 454, 44–52. https://doi.org/10.1016/j.ab.2014.03.003

Sanderson, B. A., Sowersby, D. S., Crosby, S., Goss, M., Lewis, L., & Beall, G. W. (2013). Charge density and particle size effects on oligonucleotide and plasmid DNA binding to nanosized hydrotalcite. Biointerphases, 8(1), 8. https://doi.org/10.1186/1559-4106-8-8

Tripp, J. D., Lilley, J. L., Wood, W. N., & Lewis, L. (2013). Enhancement of plasmid DNA transformation efficiencies in early stationary-phase yeast cell cultures. Yeast (Chichester, England), 30(5), 191–200. https://doi.org/10.1002/yea.2951

McKinney, J. S., Sethi, S., Tripp, J. D., Nguyen, T. N., Sanderson, B. A., Westmoreland, J. W., … Lewis, L. (2013). A multistep genomic screen identifies new genes required for repair of DNA double-strand breaks in Saccharomyces cerevisiae. BMC Genomics, 14, 251. https://doi.org/10.1186/1471-2164-14-251

Lee, C. K., Araki, N., Sowersby, D. S., & Lewis, L. (2012). Factors affecting chemical-based purification of DNA from Saccharomyces cerevisiae. Yeast (Chichester, England), 29(2), 73–80. https://doi.org/10.1002/yea.1918

Becerra, S. C., Thambugala, H. T., Erickson, A. R., Lee, C. K., & Lewis, L. (2012). Reversibility of replicative senescence in Saccharomyces cerevisiae: effect of homologous recombination and cell cycle checkpoints. DNA Repair, 11(1), 35–45. https://doi.org/10.1016/j.dnarep.2011.10.003

Lewis, L., Lee, C. K., Araki, N., & Sowersby, D. S. (2011). Factors  affecting chemical-based purification of DNA from Saccharomyces cerevisiae.

Westmoreland, J. W., Summers, J. A., Holland, C. L., Resnick, M. A., & Lewis, L. (2010). Blunt-ended DNA double-strand breaks induced by endonucleases PvuII and EcoRV are poor substrates for repair in Saccharomyces cerevisiae. DNA Repair, 9(6), 617–626. https://doi.org/10.1016/j.dnarep.2010.02.008

Lewis, L., Robson, M., Vecherkina, Y., Ji, C., & Beall, G. W. (2010). Interference with spectrophotometric analysis of nucleic acids and proteins by leaching of chemicals from plastic tubes. BioTechniques, 48(4), 297–302. https://doi.org/10.2144/000113387

Wasko, B. M., Holland, C. L., Resnick, M. A., & Lewis, L. (2009). Inhibition of DNA double-strand break repair by the Ku heterodimer in mrx mutants of Saccharomyces cerevisiae. DNA Repair, 8(2), 162–169. https://doi.org/10.1016/j.dnarep.2008.09.010

Beall, G. W., Sowersby, D. S., Roberts, R. D., Robson, M. H., & Lewis, L. (2009). Analysis of oligonucleotide DNA binding and sedimentation properties of montmorillonite clay using ultraviolet light spectroscopy. Biomacromolecules, 10(1), 105–112. https://doi.org/10.1021/bm800970v

Lewis, L., Lobachev, K., Westmoreland, J. W., Williamson, K. M., & Resnick, M. A. (2006). Use of a restriction endonuclease cytotoxicity assay to identify inducible GAL1 promoter variants with reduced basal activity.

Lewis, L., Lobachev, K., Westmoreland, J. W., Karthikeyan, G., Williamson, K. M., Jordan, J. J., & Resnick, M. A. (2005). Use of a restriction endonuclease cytotoxicity assay to identify inducible GAL1 promoter variants with reduced basal activity. Gene, 363, 183–192. https://doi.org/10.1016/j.gene.2005.09.007

Lewis, L., Karthikeyan, G., Cassiano, J., & Resnick, M. A. (2005). Reduction of nucleosome assembly during new DNA synthesis impairs both major pathways of double-strand break repair. Nucleic Acids Research, 33(15), 4928–4939. https://doi.org/10.1093/nar/gki806

Chen, L., Trujillo, K. M., Van Komen, S., Roh, D. H., Krejci, L., Lewis, L., … Tomkinson, A. E. (2005). Effect of amino acid substitutions in the rad50 ATP binding domain on DNA double strand break repair in yeast. The Journal of Biological Chemistry, 280(4), 2620–2627. https://doi.org/10.1074/jbc.M410192200

Lewis, L., Storici, F., Van Komen, S., Calero, S., Sung, P., & Resnick, M. A. (2004). Role of the nuclease activity of Saccharomyces cerevisiae Mre11 in repair of DNA double-strand breaks in mitotic cells. Genetics, 166(4), 1701–1713.

Lewis, L. (2002). “Systems for in vivo site-directed mutagenesis using oligonucleotides.”

Karthikeyan, G., Lewis, L., & Resnick, M. A. (2002). The mitochondrial protein frataxin prevents nuclear damage. Human Molecular Genetics, 11(11), 1351–1362.

Lewis, L., Karthikeyan, G., Westmoreland, J. W., & Resnick, M. A. (2002). Differential suppression of DNA repair deficiencies of Yeast rad50, mre11 and xrs2 mutants by EXO1 and TLC1 (the RNA component of telomerase). Genetics, 160(1), 49–62.

Bennett, C. B., Lewis, L., Karthikeyan, G., Lobachev, K. S., Jin, Y. H., Sterling, J. F., … Resnick, M. A. (2001). Genes required for ionizing radiation resistance in yeast. Nature Genetics, 29(4), 426–434. https://doi.org/10.1038/ng778

Storici, F., Lewis, L., & Resnick, M. A. (2001). In vivo site-directed mutagenesis using oligonucleotides. Nature Biotechnology, 19(8), 773–776. https://doi.org/10.1038/90837

Lewis, L., & Resnick, M. A. (2000). Tying up loose ends: nonhomologous end-joining in Saccharomyces cerevisiae. Mutation Research, 451(1–2), 71–89.

Lewis, L., Westmoreland, J. W., & Resnick, M. A. (1999). Repair of endonuclease-induced double-strand breaks in Saccharomyces cerevisiae: essential role for genes associated with nonhomologous end-joining. Genetics, 152(4), 1513–1529.

Lewis, L., Kirchner, J. M., & Resnick, M. A. (1998). Requirement for end-joining and checkpoint functions, but not RAD52-mediated recombination, after EcoRI endonuclease cleavage of Saccharomyces cerevisiae DNA. Molecular and Cellular Biology, 18(4), 1891–1902.

Lewis, L., Harlow, G. R., Gregg-Jolly, L. A., & Mount, D. W. (1994). Identification of high affinity binding sites for LexA which define new DNA damage-inducible genes in Escherichia coli. Journal of Molecular Biology, 241(4), 507–523. https://doi.org/10.1006/jmbi.1994.1528

Lewis, L., Jenkins, M. E., & Mount, D. W. (1992). Isolation of DNA damage-inducible promoters in Escherichia coli: regulation of polB (dinA), dinG, and dinH by LexA repressor. Journal of Bacteriology, 174(10), 3377–3385.

Lewis, L., Jenkins, M. E., & Mount, D. W. (1992). Isolation of DNA damage-inducible promoters in Escherichia coli: regulation of polB (dinA), dinG, and dinH by LexA repressor. Journal of Bacteriology, 174(10), 3377–3385.

Lewis, L., Thliveris, A. T., Ennis, D. G., & Mount, D. W. (1990). SOS Functions. In M. Riley & K. Drlica (Eds.), In The Bacterial Chromosome.